Increase the separation between the equipment and receiver. Spectrum compact achieves a 1bp resolution as can be seen in the d1s1656 and d2s441 loci in the powerplex fusion 6c system allelic ladder. Through defined and recorded can commands the system may be controlled by any suitable computer. Powerplex fusion 6c system for use on the applied biosystems. Powerplex fusion direct amplification of dna from buccal swabsforensic biology protocols for forensic str analysis status. Powerplex fusion 6c system product information the powerplex fusion 6c system is compatible with the applied biosystems 3500 and 3500xl genetic analyzers as well as applied biosystems 3 and 3xl genetic analyzers with data collection software version 4. Upon selection, the respective window for statistical analysis will open according to the specifications set by user for kit and race. Refer to the instrument user s manual for additional details. Administrators manual and operating instructions, quick reference. Documentation and resources of all forms for fusion3s line of affordable, highperformance 3d printers. Use of the powerplex fusion 6c system to amplify extracted dna. Developmental validation of the ande 6c system for rapid. Powerplex fusion and the applied biosystems globalfiler.
Powerplex fusion 6c system, by promega, is one of the most recent. For more information on the iqc markers, see chapter 5, assess the pcr reaction with the internal quality control system. View and download ness powerplex edr400 instruction manual online. The powerplex fusion 6c system is a 27locus multiplex that allows coamplification and fluorescent detection of the 18 autosomal loci in the expanded codis core loci as well as amelogenin and dys391 for gender determination. Promega powerplex fusion 5dye cc5 internal lane standard 500 fga a vwa. Pdf the powerplex fusion 6c system is a 27locus, sixdye, multiplex that. Forensic biology protocols for forensic str analysismanual appendix for powerplex fusion status. The powerplex fusion 6c system allows multiplex pcr, through simultaneous. From intelligent control units with touchscreens to automatic sensors and manual momentary. The powerplex fusion 6c system provides all materials necessary to amplify str regions of human genomic dna, including a hotstart thermostable dna polymerase, which is a component of the powerplex fusion 6c 5x master mix. Ness powerplex edr400 instruction manual pdf download. Hardware and can commands are in conformance with the sae j1939 standard.
Revised 1218 tmd045 technical manual powerplex fusion 6c system for use on the applied biosystems genetic analyzers instructions for use of products dc2705, dc2720 and dc2780. Manual set up is allowed if the qiagility is unavailable e. Powerplex fusion 6c system technical manual manualzz. However, this manual interpretation can be timeconsuming and subjective. The thermal cycling method for extracted dna samples was. Powerplex fusion 6c the largest commercial str multiplex kit available for ce use. The powerplex fusion 6c system is a 6color, 27locus multiplex for human. Powerplex fusion direct amplification of dna from buccal. Internal validation and comparative analysis of the promega. Promega corporation 2800 woods hollow road madison, wi 537115399 usa toll free in usa 8003569526 6082744330 fax 6082772516 3 tmd039 revised 417 this manual contains protocols for use of the powerplex fusion system with the geneamp pcr system 9700 and veriti 96well thermal cycler in addition to protocols to separate amplified products and detect. Selectscience trusted information for laboratory scientists. The samples were analyzed with the laboratorys at of 50 rfu. The penta d, penta e, d22s1045, tpox and se33 loci are also included to increase discrimination and allow searching of databases that include profiles with these loci.
Pdf developmental validation of the powerplex fusion 6c system. Growing digitisation makes smart onboard electrical systems in watercraft and vehicles a topical subject in global competition. Forensic biology protocols for forensic str analysis manual. Cctv power supplies, security power supplies, access control. In addition, the malespecific dys391 locus, penta d, penta e, d2s38 and. It is an ethernet transducer with fast, 1cycle updates that can be used in scada automation schemes and in synchronizing applications in conjunction with plcs. Cctv power supplies, security power supplies, access control power supplies, monitored power supplies. Initial assessment of ngs as a tool to augment routine ce. Ness powerplex edr400 instruction manual 65 pages 4 channel digital video recorder. The powerplex ii ppx ii is a new automation transducer built in the same package as the original powerplex, or with digital io added, in the m571 case. Ness powerplex edr400 manuals manuals and user guides for ness powerplex edr400. August 01, 2004 before installing and using this unit, please read this manual carefully.
A personal, affordable benchtop instrument for sanger sequencing and fragment analysis applications. Additionally, it contains two, rapidly mutating, ystrs and is capable of both casework and database workflows, including direct amplification. Bitronics powerplex ii automation transducer novatech. Powerplex is a stateoftheart solution, giving your application the smartness it deserves. Bitronics powerplex ii automation transducer novatech power. According to manufacturers manual, the recommended dna input for optimal. The samples were interpreted in strmix and lrs calculated for the known contributors. Use of the powerplexr fusion 6c system to amplify dna from. Powerplex fusion direct amplification of dna from buccal swabs. Sep 20, 2017 globalfiler is a str multiplex assay for the amplification of 24 markers, 10 of which are ministrs below 220 bp. The powerplex esi 16 system is compatible with the abi prism 310, 3100 and 3100avant genetic analyzers and applied biosystems 3, 3xl, 3500 and 3500xl genetic analyzers.
The manufacturer reserves the right to revise and improve its products. We have 4 everfocus powerplex edr1600 manuals available for free pdf download. Globalfiler and globalfiler iqc pcr amplification kits user guide for use with. Connect the equipment into an outlet on a circuit different from that to which the receiver is connected. Analysis of internal validation datasets using opensource. Validation study and forensic applications of investigator. Please make sure to keep it handy for later reference. Powerplex fusion 6c system instructions for use of products. In addition to the loci listed above, the globalfiler iqc pcr amplification kit amplifies 2 internal quality control iqc markers.
Spectrum compact ce system promega france pdf catalogs. Thermo scientific 2011 nanodrop spectrophotometer user manual, v3. The investigator 24plex qs kit user guide describes instrument setup and sample preparation of pcr products using the applied biosystems 3500. Identifiler, powerplex fusion system, powerplex fusion 6c system, and powerplex 21 system. Powerplexr fusion 6c system for use on the applied. Separation and detection were conducted with an applied biosystems 3500 genetic analyzer following lab. This software is an allinone solution for configuration, graphics production, diagnosis and simulation. The powerplex fusion 6c system is compatible with the applied biosystems 3500 genetic analyzer as reported in the technical manual 5.
Dna volumes added to pcr amplification varied up to 15. This manual contains protocols for use of the powerplex fusion system with the geneamp pcr system 9700 and veriti 96well thermal cycler in addition to protocols to separate amplified products and detect separated material on. This manual contains protocols for use of the powerplex fusion 6c system with the geneamp pcr system 9. The powerplex fusion 6c system allows coamplification and fluorescent detection of 27.
Use of the powerplexr fusion 6c system to amplify dna. This system contains sufficient reagents for 200 reactions of 25l. Powerplex fusion promega powerplex y23 promega applied genetics background on str loci and kits. We have 1 ness powerplex edr400 manual available for free pdf download. Use of the powerplex fusion 6c system to amplify dna from storage card punches. The schematic diagram illustrates the fluorescent dye label color and relative pcr product size ranges for the various str loci present in this particular kit. Optimizing dna recovery and forensic typing of degraded blood. The system contains the loci penta e, d18s51, d21s11, th01, d3s58, fga, tpox, d8s1179, vwa, amelogenin. Instructions for use of products dc2705, dc2720 and dc2780.
The powerplex 6c matrix standard was developed for use with the promega 6color powerplex. The investigator 24plex qs kit user guide describes instrument setup and sample preparation of pcr products using the applied biosystems 3500 series data collection software and the genemapper idx software, but an internal validation is always needed. Manuals and user guides for everfocus powerplex edr1600. Powerplexr fusion system for use on the applied biosystems. Se33, penta d and penta e 3 ystr markers dys391, dys570, dys576 a one kit for both direct amplification and casework with a 60 min pcr time capability.
Technical manual powerplex fusion 6c system for use on the. Refer to the 3500 series data collection software user manual for the criteria recommended when. The powerplex fusion 6c system is a new 6dye, 27locus, str multiplex. All data described in this manuscript were generated with components from the commercial release of the powerplex fusion 6c system. Aug 14, 2019 promega 2017 powerplex fusion 6c system for use on the applied biosystems.
Powerplex fusion 6c is a 27locus multiplex assay that has the same markers as globalfiler, including 8 autosomal ministrs plus some additional markers to increase the discrimination power. Unless noted otherwise, protocols in the powerplex fusion 6c system technical manual were followed. Has a total of 27 loci including the 20 codis core loci. The powerplex fusion 6c system is a sixdye chemistry. Comparison of four commercial qpcr kits for analyzing. The penta d, penta e, d22s1045, tpox and se33 loci are also included to increase discrimination and allow searching of databases that.
Document includes user manual fusion 5 user manual. Str loci and multiplex kits becky hill research biologist, applied genetics group. L and used 29cycles as described in the technical manual. The effect of different levels of degradation and dna. The information in this manual was current when published. Developmental validation of the powerplex fusion 6c system. The powerplex fusion 6c system is a 27locus, sixdye, multiplex that includes all markers in the expanded codis core loci and increases overlap with str database standards throughout the world.
Refer to the 3500 series data collection software user manual. From intelligent control units with touchscreens to automatic sensors and manual momentary switches or smartphone and tablet applications powerplex puts the entire onboard electrical system under your thumb. Tablet pc user manual details for fcc id 2aikxfusion5w104 made by f5cs ltd. Amplification kits globalfiler and globalfiler iqc pcr. The primer set and the allelic ladder in the globalfiler kit are different. Reagent as recommended in the swabsolution kit technical manual.
1189 26 822 1381 498 1214 643 1428 103 1200 359 85 830 535 1411 1255 1559 59 46 452 948 1397 453 1162 1466 226 983 988 1196 1412 997 1129 1469 951 1043 1119 409